The use of degenerated primers in DNA barcoding has been a topic of debate for quite some time. A recent study conducted in 2024 suggests that the use of degenerated primer may significantly affect the accuracy of COX1 barcoding. This finding raises questions about the reliability of existing DNA barcoding data and the potential impact on biodiversity research. Scientists and researchers must critically evaluate the use of degenerated primers to ensure the validity and reproducibility of DNA barcoding results.

Read Full Article Here

source: Phys.org